Fig. 2.

BCL-2 inhibition inhibits proliferation and induces apoptosis of OTSCC cells. a Bcl-2 protein expression level on BCL-2 knockdown by siRNA in SCC-25, SCC-9, CAL27 and Tca3188 cells. b Proliferation is decreased and c apoptosis is increased in Bcl-2 depleted OTSCCs. Cells are electroporated with 100 nM siRNA control or siRNA BCL-2 and cultured for 48 h prior to WB, proliferation and apoptosis assays. ABT-199 inhibits proliferation (d) and induces apoptosis (e) of OTSCCs in a dose-dependent manner. Data were presented as mean ± SD. *p < 0.05