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Relative C = C cleavage activity (conversion %) on α-methylstyrene (based on acetophenone formation) of heat-purified TM1459 and variants thereof (1 mg/mL). Reaction conditions: 50 mM α-methylstyrene and 150 mM t-BuOOH in 15% ethylacetate in 50 mM NaPi, pH 7.0, 30°C and 1,000 rpm for 14 h. 100% refers to conversion of TM1459 (30% conversion).
