FIGURE 7.

Time course of the product formation of TM1459 (1 mg total protein) wild type (black) and -C106Q (gray, full line), -I49A/F94A (gray, dashed line), and -C106V/L107^∗ (gray, dotted line) over the first 2 h of the bioconversion reaction with 50 mM α-methylstyrene and 150 mM t-BuOOH in 15% ethylacetate in 50 mM NaPi, pH 7.0, 30°C and 1,000 rpm. The low product concentration obtained with the wild type in the first 30 min of the reaction accounts for lower accuracy in the quantification, resulting in an apparent non-continuous time profile.