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. 2016 Sep 22;9:88. doi: 10.3389/fnmol.2016.00088

FIGURE 5.

FIGURE 5

Effects of 100 μM ZX1 on IPSCs mediated by α1β GlyRs. (A) Superimposed averaged, normalized IPSCs each averaged from >50 events from the same cell. Recordings were made in the presence of control solution (black trace; averaged from 79 events), after addition of 100 μM ZX1 (red trace; averaged from 61 events) and after 5 min washout (pink trace; averaged from 52 events). (B–D) Effects of 100 μM ZX1 on IPSC amplitude, decay time constant and 10–90% rise time for α1β-mediated IPSCs. All results were averaged from 10 cells. Statistical significance in (D) was determined by Friedman test with significance represented by ∗p < 0.05, and ∗∗∗∗p < 0.0001. (E–G) Comparison of the effects of 10 mM tricine and 100 μM ZX1 on IPSC amplitude, decay time constant and 10–90% rise time for α1β-mediated IPSCs. There was no significant difference in the decay time constants in either condition. Statistical significance in (G) was determined by two-tailed Wilcoxon matched-pairs signed rank test, with significance represented by ∗∗p < 0.01 and ∗∗∗p < 0.001. (H) Time course of washout of the ZX1 effect on decay time constant. ZX1 was applied for 3 min. All data points were averaged from five cells.