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. 2016 Sep 14;283(1838):20161346. doi: 10.1098/rspb.2016.1346

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Samples of coleoid microspectrophotometric measurements using different mountants (I. notoides). (a) A sample of the MSP retinal preparation seen via an infrared image converter. Arrowhead indicates the light beam; S, screening pigment granules; Rh, rhabdominal layer. Scale bar, 20 µm. (b–d) Mean pre-bleached (open grey square) spectra and post-bleached spectra (dark grey triangle) in different concentrations of hydroxylamine solution. Pre-bleached spectra are overlaid with the best-fit rhodopsin template (black line). (e) Changes of the paired spectral curves in the standard mountant (λ_max shift from 492 to 494 nm). (f) Changes of the paired spectral curves in the alkaline mountant (λ_max shift from 492 to 380 nm). (g) λ_max differences between ESP and MSP results (mean ± 1 s.d.) in four coleoid groups. The capital E indicates λ_max measured by ESP; M indicates that using MSP; n indicates the number of species.