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. 2016 Sep 22;10:427. doi: 10.3389/fnins.2016.00427

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Copyright © 2016 Althobaiti, Alshehri, Almalki and Sari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of METH (10 mg/kg i.p. every 2 h × 4), ethanol and CEF (200 mg/kg) on GLT-1 expression in the NAc and PFC. (A,C) Immunoblots for GLT-1 as well as β-tubulin, which was used as a control loading protein, in the NAc and PFC, respectively, as compared to water-pretreated groups and ethanol-pretreated groups. (B,D) Quantitative analysis revealed a significant increase in the ratio of GLT-1/β-tubulin in METH-CEF-treated (WMC or EMC) rats compared to the METH-Saline-treated rats in the water (WM) and the ethanol (EM) groups, in the NAc and PFC, respectively. Significant downregulation of GLT-1 expression was revealed in the METH-Saline-treated groups compared to control in water- and ethanol-treated groups in the NAc and PFC. Significant downregulation of GLT-1 expression was revealed in ethanol-Saline-Saline (ES) and ethanol-METH-Saline (EM) groups compared to its corresponding water control groups in the NAc, but not in the PFC. No significant difference in GLT-1 expression was revealed in water-METH-CEF-treated (WMC) rats compared to water control groups. However, a significant increase in GLT-1 expression was found in the Ethanol-METH-CEF (EMC) group compared to ethanol control (ES) group in the NAc, but not in the PFC. ^*p < 0.05, ^**p < 0.01 (or &&, for comparison between ethanol and its corresponding water control groups), ^***p < 0.001, and ^****p < 0.0001. Values shown as means ± S.E.M. n = 6 for each group.