Table 1.

Effect of different treatments on spontaneous locomotor activity in mice

Treatment		Activity counts/4 min	n	p value
acute	1 % Tween 80	1209.0 ± 92.6	10
	sildenafil 30 mg/kg	974.9 ± 107.0	11	0.237
	sildenafil 60 mg/kg	972.7 ± 132.0	9
subchronic	1 % Tween 80	1191.0 ± 46.6	11
	sildenafil 30 mg/kg	1283.0 ± 66.2	12	0.366
	sildenafil 60 mg/kg	1324.0 ± 77.4	12
acute	1 % Tween 80 + 1 % DMSO	1083.0 ± 101.5	10
	1 % Tween 80 + reserpine (2 mg/kg)	77.1 ± 21.1	12	< 0.001
	sildenafil 60 mg/kg + reserpine (2 mg/kg)	65.67 ± 22.7	12	< 0.001
subchronic	1 % Tween 80 + 1 % DMSO	1192.0 ± 94.9	12
	1 % Tween 80 + reserpine (2 mg/kg)	220.8 ± 73.5	12	< 0.001
	sildenafil 60 mg/kg + reserpine (2 mg/kg)	360.1 ± 63.6	12	< 0.001
acute	1 % Tween 80	1134.0 ± 88.6	12
	1 % Tween 80 + restraint stress	786.3 ± 64.0	11	< 0.01
	sildenafil 60 mg/kg + restraint stress	708.4 ± 73.7	11	< 0.01
subchronic	1 % Tween 80	963 ± 64.9	12
	1 % Tween 80 + restraint stress	968.3 ± 94.7	12	0.315
	sildenafil 60 mg/kg + restraint stress	1126 ± 91.5	12

Data are presented as means ± SEM. In acute studies, sildenafil was injected 30 min before the test. In subchronic studies, sildenafil was administered once daily for 14 consecutive days and the locomotor activity test was performed 24 h after the last injection. Data were analyzed with one-way ANOVA followed by Tukey post hoc test