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Protein analyses of IL-36 (α, β, and γ), IL-36R, and IL-36Ra. Uterus extracts were tested with antibodies against IL-36α, β, γ, IL-36R, and IL-36Ra. Actin was used as a loading control. (A) Western blot of representative data from four individuals. (B–F) Protein levels are expressed as fold increases normalized to GAPDH and relative to diestrus. d, diestrus; p, proestrus; e, estrus; m, metestrus; dpc, days post coitum; L, labor; dpl, days post-labor.
