Table 1.
pH and concentrations of metabolites (mM) in culture supernatant measured with HPLC after 24 h incubation of mono- and co-cultures of Bifidobacterium pseudolongum PV8-2 in CSDLIM media (mean ± SD, n = 3).
| B. pseudolongum PV8-2 | Acetate | Lactate | Formate | pH |
|---|---|---|---|---|
| with iron supplementation | ||||
| B. pseudolongum PV8-2 | 20.81 ± 0.35 | 8.04 ± 0.34 | 3.85 ± 0.23 | 4.50 ± 0.06 |
| S. Typhimurium N15 | 5.26 ± 0.09 | 14.82 ± 0.26 | - | 4.87 ± 0.02 |
| B. pseudolongum PV8-2/S. Typhimurium N15 | 21.81 ± 0.82 | 10.15 ± 0.57 | 3.20 ± 0.30 | 4.19 ± 0.02 |
| E. coli O157:H45 | 5.55 ± 0.07 | 12.72 ± 0.21 | - | 4.88 ± 0.01 |
| B. pseudolongum PV8-2/E. coli O157:H45 | 21.90 ± 0.75 | 9.87 ± 0.30 | 4.79 ± 0.21 | 4.15 ± 0.01 |
| with iron supplementation (30 μM) | ||||
| B. pseudolongum PV8-2 | 23.28 ± 0.36* | 9.43 ± 0.20* | 4.96 ± 0.75* | 4.20 ± 0.01* |
| S. Typhimurium N15 | 5.47 ± 0.28 | 14.07 ± 0.56 | - | 5.16 ± 0.06 |
| B. pseudolongum PV8-2/S. Typhimurium N15 | 21.92 ± 0.72 | 10.64 ± 0.36 | 2.53 ± 0.25 | 4.21 ± 0.02 |
| E. coli O157:H45 | 5.93 ± 0.14 | 11.87 ± 0.09 | - | 5.05 ± 0.02 |
| B. pseudolongum PV8-2/E. coli O157:H45 | 22.81 ± 0.82 | 9.73 ± 0.43 | 4.82 ± 0.33 | 4.18 ± 0.01 |
Stars (*) denote a significant (P < 0.05) difference in the respective metabolite production of cultures performed in iron supplemented compared with unsupplemented medium.