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. 2016 Sep 8;2016:8263463. doi: 10.1155/2016/8263463

Figure 2.

Figure 2

A representative UHPLC-QDA chromatogram quantifying hesperidin in cerebral supernatant of rats. Peaks observed at a retention time of 5.6 min and 6.3 min for hesperidin and diosmin are in accordance with the standards, respectively. Panels (a) and (b) show the chromatograms of control samples from sham and vehicle groups. Chromatograms of hesperidin at acute dosages of 10 mg/kg, i.p. at 30 min (c) and 120 min (d); 100 mg/kg, i.p. at 30 min (e) and 120 min (f); and 1000 mg/kg, i.p. at 30 min (g) and 120 min (h). Mass spectrum describing the molecular weight of diosmin (i) and hesperidin (deprotonated molecular ion [M-H]−) (j) and the corresponding retention times.