Skip to main content
. 2016 Sep 8;2016:3939540. doi: 10.1155/2016/3939540

Figure 3.

Figure 3

Q7 produces oxidation of lipids and decreases endothelial NO. Depicting oxidative stress revealed by Q7-mediated lipid oxidation and a change of endothelial NO release in rat aorta tissue. The rat aorta homogenate determination of TBARS in presence of Q7 (10−6 and 10−5 M) was measured as % with respect to control (a). Significant differences were found between 10−5 M Q7 versus control or 10−6 M of Q7 (∗ p < 0.05). The production of NO by a segment of the rat aorta was measured by the accumulation of nitrite using Griess reaction method (b): the aortic rings were incubated with vehicle (control), 10−5 M ACh, 10−5 M Q7 plus 10−5 M ACh, and 10−5 M ACh plus 10−4 M L-NAME. Data are the average ± SEM of 5 independent experiments. ∗∗∗ p < 0.001 versus control; ### p < 0.001 versus ACh; effect of Q7 on eNOS activity and production of endothelial NO (c). The production of NO was determined indirectly by the formation of L-citrulline in the reaction catalyzed by eNOS in HUVEC. The results are mean standard error of the mean. Asterisk indicates statistically significant differences compared to the control (∗ p < 0.05; n = 3).