Figure 1.

Human pancreatic cancer associated fibroblasts activate Ets-2 signaling.

(A) Dual-color IHC (ETS2, brown; SMA, red) of human normal or PDAC Tissue Microarray. Insets show higher magnified view of representative areas. Black arrows indicate ETS2+/SMA+ fibroblasts. Scale bars 25 μm. (B) Quantification of ETS2 staining in SMA+ positive cells shown in A (red bar indicates mean). (C) qRT-PCR analysis of proto-oncogenic transcription factors in pancreatic fibroblasts isolated from normal or PDAC patients (normalized to Rpl4, represented as fold-change relative to normal fibroblast expression; n = 3; bars represent means ± Standard Deviation [SD]).