Supplemental Figure 1.
(A) Graphic of inherited alleles in epithelial and fibroblast cellular lineages. (B) Ets2 genotyping PCR of DNA derived from formalin-fixed, paraffin-embedded tissue of indicated genotypes. (C) Dual-color IHC (ETS2, brown; SMA, red) of KE and KCE pancreata (E = epithelium; S = stroma). Scale bars 25 μm. (D–E) Quantification of ETS2 staining in SMA+ positive fibroblasts or pancreatic epithelium shown in A (red bar indicates mean; n = 7). (F) Co-IF for F480/ETS2; white arrows indicate F480/ETS2 dual-positive cells. (G) Quantification of ETS2 staining in F4/80+ positive cells shown in F (red bar indicates mean; n = 3). (H) Co-IF of pancreatic fibroblast cultures stained for SMA/CK19 or vimentin/CK8. (I) qRT-PCR of Ets-2 in KE and KCE mouse fibroblast cultures (normalized to Rpl4, represented as fold-change relative to ETS2WT expression; n = 3; bars represent means ± SD). (J) Ets2 genotyping PCR of DNA derived from cultured fibroblasts of indicated genotypes.