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Number of iPSC Clones Isolated, Expanded, and Characterized Following Targeting of the DNMT3B Locus
| Targeted (EGFP+) Clones |
Non-targeted (EGFPneg) Clones | Total Clones Analyzed | ||
|---|---|---|---|---|
| One Allele | Both Alleles | |||
| SpCas9 | 17 (0) | 11 | 25 | 53 |
| SpCas9-Gem | 22 (8) | 6 | 23 | 51 |
| SpCas9-Cdt1 | 16 (2) | 1 | 22 | 39 |
The number of clones with EGFP targeted to one allele without an additional mutation in the untargeted allele is shown in parentheses.
