Figure 5.
Lineage Tracing of K14+ Epithelial Progenitors in the K14CreERT2-Confetti Transgenic Mouse Meibomian Gland at 23 and 59 Weeks Post Tamoxifen Injection of a 10-Week-Old Mouse
(A) Individual acini are completely labeled with one color, suggesting that they are renewed from a single, independent lineage when compared with other acini and the ductal epithelium.
(B–E) K6 immunostaining confirms that acinar confetti labeling is separated from the ductal epithelium and suggests that there are distinct lineages for acini and ductal epithelium. Meibomian gland acinar labeling is analogous to the labeling of the sebaceous gland (C). Confetti labeling in a 200-μm vibratome section (D) and a 10-μm cryosection (E) also showed labeling in the ductal epithelium that is separate from the lineages stochastically labeled in the acini. Ac, acinus; Du, duct; HF, hair follicle.