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. 2016 Aug 11;7(3):399–410. doi: 10.1016/j.stemcr.2016.07.010

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© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

High-resolution 3D Reconstruction of the K14CreERT2-Confetti Mouse Eyelid after 59 Weeks Post Tamoxifen

(A) Fluorescence imaging of labels generated by stochastic recombination in the K14CreER^T2-Confetti mouse meibomian gland after 59 weeks verified that acini were entirely composed of a single color (RFP) or two-color combinations (RFP/GFP).

(B–D) A high-resolution 3D reconstruction of lineage tracing in the K14CreER^T2-Confetti mouse illustrates that (C) acini were independently labeled and (D) the duct comprised more than a single lineage. Dashed outlines encircle the entire meibomian gland structure.