Figure 3. Characterization of HEK293 clones expressing CBP candidates.

(A) HEK293 clones expressing the five CBP candidates as chimera with EYFP were visualized by transmitted light, wide field EYFP fluorescence (green) and TIRF (red). Merged images indicate the membrane expression of the EYFP-fusion proteins. Bar = 10 μm. (B) The clones were subjected to static adherence in 96-well plates coated or not (control) with 25 pmoles of CX3CL1 or CCL2 per well, as indicated. The number of adherent cells per mm² was expressed as mean values and standard deviations from four replicates wells. ANOVA followed by post hoc analysis with Tukey test was performed to establish the levels of significance: ***p ≤ 0.0005.