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. 2016 Sep 22;6:33786. doi: 10.1038/srep33786

Figure 7. Specific and functional interaction between CX3CL1 and the CBP-EC peptides assayed by fluorescence and static adherence.

Figure 7

(A) Fluorescence of each of the 5-TAMRA fluorescent peptides were measured in the presence or not of CCL2 or CX3CL1 (excitation 520 nm, emission 580 nm). Experiments are made in triplicate. The background (fluorescence of the control without chemokine) was subtracted and the data were expressed as mean values and standard deviations from three independent experiments. **p ≤ 0.005; ***p ≤ 0.0005. (B) Static adherence in 96-well plates coated with 25 pmoles of CX3CL1 of enriched 3D7-iRBC pretreated or not (control) with 0.1 μM of various peptides as indicated. The data are expressed as percent of the control, as mean values and standard deviations from four independent experiments. ANOVA followed by post hoc analysis with Tukey test was performed to establish the levels of significance: **p ≤ 0.005; ***p ≤ 0.0005; not significant (ns): p > 0.05.