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. 2016 Sep 22;6:33813. doi: 10.1038/srep33813

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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A maximum likelihood analysis of GSTs detected in the A. glabripennis (ALB), mountain pine beetle (MPB), and Tribolium castaneum (red flower beetle) genomes was performed using the program Garli. The LG + I evolutionary model was selected as the optimal rate matrix using ProtTest and 500 bootstrap pseudoreplicates were analyzed. The genes were assigned to clades based on relatedness to red flour beetle and mountain pine beetle GSTs, which had been classified previously⁸. Brown circles denote GSTs that were induced by feeding in sugar maple, while grey circles denote GSTs that were more strongly induced in the diet-fed larvae.