Table 5. Bioinformatics analysis of high throughput sequencing data from selection rounds.

	Initial library	HeLa-CCR7 cell-SELEX				HeLa-CD2 cell-SELEX
		Round 3	Round 5	Round 6	Round 7	Round 3	Round 5	Round 6	Round 7
Total Reads	40,174,712	57,383,383	49,925,077	57,778,216	51,453,049	15,346,437	16,615,193	18,644,363	21,236,560
Usable reads	37,951,292	54,654,814	45,474,572	51,503,799	44,593,424	13,682,508	14,585,023	16,629,267	19,596,020
% Usable	94.47%	95.25%	91.09%	89.14%	86.67%	89.16%	87.78%	89.19%	92.27%
Total reads of top 1000 unique sequences	3,169	5,797	226,559	564,840	771,042	326,175	6,766,517	9,237,787	16,182,954
Frequencies of top 1000 in all the usable reads	0.008%	0.011%	0.498%	1.097%	1.729%	2.384%	46.394%	55.551%	82.583%
Molecular enrichment (fold) (top 1000)	1.00	1.83	71.49	178.24	243.31	102.93	2135.22	2915.05	5106.64

The total reads and useful reads were defined. The most frequent 1,000 unique sequences and their percent in all the usable reads were identified. The molecular enrichment at each round was calculated by the formula: total reads of top 1000 unique sequences at round X/unselected round (initial library).