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. 2016 Sep 22;6:33830. doi: 10.1038/srep33830

Figure 2. Increase of LC3 protein expression in RGCs and their axons of aged E50K−tg mice.

Figure 2

(a) Immunoblot analysis of LC3 protein in retinal extracts from 16-month-old aged E50K−tg mice. For each determination, the actin level in age-matched WT mice was normalized to a value of 1.0. Data are shown as the mean ± S.D. (n = 3 independent experiments). *P < 0.05 compared with the WT group. Full-length blots are presented in Supplementary Figure 6. (b) Immunohistochemical analysis of LC3 protein expression in retinal sections from WT and E50K−tg mice. Both RGC somas and axons increased LC3 immunoreactivity in E50K−tg mice. Arrows indicate LC3-positive RGC somas in the GCL and arrowheads indicate LC3-positive RGC axons in the GCL. E50K−tg, E50K mutation-carrying transgenic; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; LC3, microtubule-associated protein 1A/1B-light chain 3; OPL, outer plexiform layer; OPTN, optineurin; RGC, retinal ganglion cell; WT, wild-type. Scale bar, 20 μm.