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. 2016 Sep 22;6:33836. doi: 10.1038/srep33836

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Copyright © 2016, The Author(s)

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Sagittal sections of the control, cavity and C + hMSCs were stained with (A) H&E and (B) SO-FG. One representative donor was depicted. In the IVD whole section (scale bar: 2000 μm), CEP, NP and AF regions of IVD can be distinguished. Magnifications of NP/AF cells are presented (scale bar: 20 μm) in the H&E staining as well as magnifications of the whole AF/NP tissue in SO-FG (scale bar: 200 μm). Collagens are visualized by green/blue (arrow) color while GAG are observed in orange color. A higher intensity of orange color in the C + hMSCs group NP suggested a higher proteoglycan content in the tissue of this experimental group.