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. 2016 Sep 22;6:33836. doi: 10.1038/srep33836

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The NP tissue of the three experimental groups was digested and afterwards analyzed for GAG and Col type II content. (A) Fold increase in GAGs content. GAG content was similar between the groups (n = 4). (B) Fold increase in Col type II and a representative image of a blot. Col type II content was determined by WB and values normalized to the total protein and presented as fold increase to the cavity group; a slight increase in Col type II was observed in the C + hMSCs group compared with the cavity (no statistically significance in raw data, Mann-Whitney test (n = 7)). Results are presented as M/D.