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. 2016 Sep 22;6:34013. doi: 10.1038/srep34013

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Copyright © 2016, The Author(s)

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(A) PCR cloning of a partial DjOB1 sequence base on Unigene 34129. (B) RT-PCR analysis after two rounds of dsRNA feeding. DjPCNA and DjOB1 were analysed, and DjEF2 was used as an internal control. (C) Phenotypes of Dugesia japonica after two rounds of dsRNA feeding. (D) Statistical quantification of the proportion of abnormal flatworms in Dugesia japonica after RNAi DjOB1. The planarians with injuries on the head, pharynx or both were analysed.