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. 2016 Sep 22;6:34056. doi: 10.1038/srep34056

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Copyright © 2016, The Author(s)

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(a) (upper panel) NOR-1 (white bars) and cIAP2 (black bars) mRNA levels analyzed by real-time PCR in VSMC exposed to hypoxia (0.2% O₂) for 4 hours (n = at least 5), *P < 0.001 vs. cells maintained in normoxia. (lower panels) Representative western blot showing protein levels of NOR-1 and cIAP2 in VSMC exposed to hypoxia for 6 hours. Levels of β-actin are shown as a loading control. (b) Immunofluorescence confocal microscopy analysis showing cIAP2 (green), and nuclei (Hoechst stain, blue) in VSMC exposed to hypoxia for 6 hours. Bars: 25 μm. (c) NOR-1 (white bars) and cIAP2 (black bars) mRNA levels analyzed by real-time PCR in VSMC exposed to hypoxia for increasing times. (d) CoCl₂ is a stronger inducer of NOR-1 and cIAP2 expression than hypoxia. mRNA levels of NOR-1 and cIAP2 analyzed by real-time PCR in VSMC exposed to hypoxia (Hyp) or CoCl₂ (0.5 mM) for 4 hours (n = at least 6). *P < 0.0001 vs. cells maintained in normoxia (Norm).