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. 2016 Sep 22;6:33948. doi: 10.1038/srep33948

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The top diagram shows the parental vector pWK3R, with the hatched arrow representing the genetic elements responsible for bFGF expression. The four constructs: (A) pWK3R, and its three derivatives: (B) pWK3ROmpAd, (C) pWK311R, and (D) pWK311ROmpAd, together with the genetic elements forming their own hatched arrows are specified underneath. Symbols for the components shown in pWK3R and its three derivatives are: ori = origin of replication in E. coli; amp^R = structural gene conferring resistance to ampicillin; ytl2-incR = ytl2-incR system for plasmid stability of Salmonella typhimurium; bfgf = bfgf gene; egf = egf gene; lacUV5 = lacUV5 promoter; lacO = lac operator; RBS = consensus ribosomal binding site; ompAL = ompA leader sequence; vma = VMA coding sequence. Arrow indicates the direction of transcription. Information in parenthesis indicates the plasmid size.