Figure 4. Expression of bFGF by transformants JM101 [pWK311ROmpAd] and JM101 [pWK3R] cultivated in a small-scale fermentor under fed-batch conditions without induction.

Culture samples of (A) JM101 [pWK311ROmpAd] and (B) JM101 [pWK3R] were taken at different time points from the fermentor and viabilities of plasmid-free and plasmid containing cells were determined on plain agar plates (---▴---) and agar plates supplemented with ampicillin (^___▪^___), respectively. CFU refers to colony-forming units. Levels of bFGF detected in the cell lysate (^___♦^___) and culture supernatant samples () are presented. The fermentation intervals present developments of cell growth and bFGF expression during two different stages of cultivation (partitioned by the vertical dotted line): last phase of glucose feeding (3–6 h) and the “pending period” (6–13 h). Each growth experiment of the two transformants was repeated three times and standard error bars are shown.