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. 2016 Sep 22;6:33948. doi: 10.1038/srep33948

Figure 5. Western blot analysis of bFGF expressed by transformants JM101 [pWK3R] and JM101 [pWK3ROmpAd] grown under shake flask and fermentation conditions.

Figure 5

Shake flask (SF) and fermentation (F) conditions for growth of JM101 [pWK3R] and JM101 [pWK311ROmpAd], respectively, are described in Methods. Culture supernatant (SN) and cell lysate (CL) samples were prepared from the former (lanes 3–4) and latter (lanes 5–10) cultures and analyzed for bFGF activities by western blotting. The amounts of SN and CL samples loaded were equivalent to 6 μl and 2.5 μl of each culture, respectively. The constructs concerned are indicated beneath the blot. The three time points: 9 h, 11 h and 13 h, at which samples collected from the JM101 [pWK311ROmpAd] culture are denoted. Other symbols used are: M = protein markers (lane 1) in kDa; +VE = bFGF standard (lane 2).