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. 2016 Sep 22;6:33948. doi: 10.1038/srep33948

Figure 6. Viable cell counts obtained from the time course study of various E. coli cultures.

Figure 6

Samples of E. coli cultures harboring the four constructs: pWK3R, pWK3ROmpAD, pWK311R and pWK311ROmpAD grown under IPTG induction were taken and assayed for plasmid-free and plasmid containing cells by differential plating. Viable cell counts obtained on plain agar plates (------) and agar plates supplemented with Amp (_________) are shown; CFU refers to colony-forming units. The names of the transformants are shown above each graph for indication of the cultures. Each assay was repeated three times (SEM < 0.1). Note that constructs pWK3R and pWK311R were equipped with the ompA signal sequence for secretory production of EGF and bFGF, whereas constructs pWK3ROmpAD and pWK311ROmpAD were not. The viable cell counts of the latter two cultures were about 10-fold higher than those of the former ones.