Table 2. Oligos used in the study.
Primer | Sequencea |
---|---|
P1 | 5′-CACTGAAACGCACTGGGCAG-TATAAACTTGGATCCAA-3′ |
P2 | 5′-ACCCTTGGCAAAGCAGCTCTTAGCAGACAT-TATAAACTTGGATCCAAAACAG -3′ |
P3 | 5′-ATGTCTGCTAAGAGCTGCTTTGCCAAGGGT-3′ |
P4 | 5′-TTTCTGCCCAGGTCCTGTTTT-GGATCCAAGTT-3′ |
P5 | 5′-ACGAGGCCCTTTCGTCTTCA-AGAATTCGCAT-3′ |
P6 | 5′-CAGAGTCACTATTCATAATTTTTTC-3′ |
P7 | 5′-GAAAAAATTATGAATAGTGACTCTG-3′ |
P8 | 5′-CAACAACACAGTTGCATGCATACTT-3′ |
aOligo sequences presented in italics are bfgf unrelated sequences containing restriction sites downstream from the designed PCR primers. Restriction sites on the italic sequences are underlined.