Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 22;6:32445. doi: 10.1038/srep32445

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Confirmation assays: (A) Retransformation analysis, (B) β-galactosidase assay. (A) Each of the 28 putative interactor proteins was used with SRBSDV-P10 protein to cotransform yeast strain NMY51 cells to confirm the interaction and eliminate false positives; pDHB1-LargeT + pDSL-p53 (positive control), pDHB1-LargeT + pPR3-N-empty prey insert (negative control). (B) β-galactosidase assay to identify the strength of interaction between SRBSDV-P10 protein and each of the 28 putative proteins. (+) = Positive control, (−) = Negative control. (1) NADH dehydrogenase subunit 1, (2) polyubiquitin, (3) alpha tubulin 84B, (4) growth hormone-inducible transmembrane protein, (5) longwave opsin, (6) cytochrome B, (7) sugar transporter 2, (8) vesicle-associated membrane protein 7, (9) sugar transporter 6, (10) ATP synthase lipid-binding protein, (11) nascent polypeptide-associated complex subunit alpha, (12) cuticlin-1, (13) ATP citrate lyase, (14) titin, (15) glucosyl glucuronosyl transferases, (16) tetraspanin 39D, (17) coronin-1C, (18) atlastin, (19) calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type, (20) integral membrane protein 2B, (21) integrin beta-3, (22) vesicle transport V-SNARE protein Vti1A, (23) tubulin beta-2C chain, (24) cytochrome C oxidase subunit III, (25) cathepsin L, (26) carboxylesterase, (27) conserved hypothetical protein, (28) vitellogenin.