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. 2016 Sep 22;6:32445. doi: 10.1038/srep32445

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Copyright © 2016, The Author(s)

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Lane 1, His-tag fusion protein (Protein-His) as input; lane 2, GST protein with His-tag fusion protein (negative control); lane 3, GST fusion protein (P10-GST) with His-tag fusion protein. The size of the His tag fusion protein for VAMP7 was (24.7 kDa + 15 kDa) 39.7 kDa, the His tag fusion protein for Vti1A was (25.4 kDa + 15 kDa) 40.4 kDa, the His tag fusion protein for Ghitm was (36 kDa + 15 kDa) 51 kDa, the His tag fusion protein for nascent polypeptide-associated complex subunit alpha was (22.5 kDa + 15 kDa) 37.5 kDa, and the His tag fusion protein for ATP synthase lipid-binding protein was (14.4 kDa + 15 kDa) 29.4 kDa.