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. 2016 Sep 22;6:32445. doi: 10.1038/srep32445

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Copyright © 2016, The Author(s)

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(A) Lane 1: Protein ladder. 2: VAMP7-His (39.7 kDa) (Input). 3: VAMP7-His + GST (Pull-down = Negative control) (GST = 27 kDa). 4: VAMP7-His + P10-GST (Pull-down) (P10-GST = 89.64 kDa) (VAMP7-His = 39.7 kDa). 5: Free lane. 6: Vti1A-His (40.4 kDa) (Input). 7: Vti1A-His + GST (Pull-down = Negative control) (GST = 27 kDa). 8: Vti1A-His + P10-GST (Pull-down) (P10-GST = 89.64 kDa) (Vti1A -His = 40.4 kDa). 9: Free lane. 10: Ghitm-His (51 kDa) (Input). 11: Ghitm-His + GST (Pull-down = Negative control) (GST = 27 kDa). 12: Ghitm-His + P-10-GST (Pull-down) (P10-GST = 89.64 kDa) (Ghitm-His = 51 kDa). (B) Lane 1: Protein ladder. 2: Nascent polypeptide-associated complex subunit alpha-His (37.5 kDa) (Input). 3: Nascent polypeptide-associated complex subunit alpha-His + GST (Pull-down = Negative control) (GST = 27 kDa). 4: Nascent polypeptide-associated complex subunit alpha-His + P10-GST (Pull-down) (P10-GST = 89.64 kDa). 5: Free lane. 6: ATP synthase lipid-binding protein-His (29.4 kDa) (Input). 7: ATP synthase lipid-binding protein-His + GST (Pull-down = Negative control) (GST = 27 kDa). 8: ATP synthase lipid-binding protein-His + P10-GST (Pull-down) (P10-GST = 89.64 kDa).