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. 2016 Sep 22;6:33307. doi: 10.1038/srep33307

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) After determining the types of cultured rat PSN by whole-cell voltage-clamp recordings by anandamide (30 μM) and capsaicin (500 nM) application, 76 cells were collected for single-cell PCR. 10 COR type, 11 ACR type and 8 “anandamide- and capsaicin non-responding” neurons (NR) were successfully processed to find out whether the neuron expressed the CB1 receptor. The sizes of the amplicons generated to specific primers for CB1 receptor (127 bp) or the house-keeping molecule GAPDH (380 bp) were indistinguishable from the predicted sizes as it is shown by these typical gel images. L indicates the size marker. Note the presence of the GAPDH and the lack of CB1 amplicon in a COR neuron. (B) All the ACR cells and 8 of 10 COR neurons express the CB1 receptor in this sample of neurons. In addition 50% of the non-responding cells also express the CB1 receptor.