| 1 |
Whole-cell recordings from rat cultured PSN in control bath solution |
39 |
29 |
10 |
From No 2: p = 0.82 From No 3: p = 0.65 From No 5: p = 0.1 From No 6: p = 0.36 From No 7: p = 0.009 From No 9: p = 0.28 From No 10: p = 0.4 From No 11: p = 1 From No 12: p = 0.08 From No 15: p = 1 |
| 2 |
Ca2+ imaging on rat PSN cultures |
57 |
40 |
17 |
From No 1: p = 0.82 From No 3: p = 1 From No 9: p = 0.07 From No 10: p = 0.11 |
| 3 |
Ca2+ imaging in WT-TRPV1 mouse PSN cultures |
54 |
37 |
17 |
From No 1 p = 0.65 From No 2: p = 1 |
| 4 |
Ca2+ imaging in TRPV1−/− mouse PSN cultures |
0 |
n/a |
n/a |
n/a |
| 5 |
Whole-cell recordings from IB4+ and IB4- cells |
25 |
13 |
12 |
From No 1: p = 0.1 From No 6: 0.76 From No 12: p = 0.8 |
| 6 |
Whole-cell recordings from rat cultured PSN in the presence of rimonabant |
18 |
11 |
7 |
From No 1: p = 0.36 From No 5: p = 0.76 From No 12: p = 0.79 |
| 7 |
Ca2+ imaging in WT-CB1 mouse PSN cultures |
124 |
113 |
11 |
From No 1: p = 0.009 From No 2: p = 0.0005 From No 3: p = 0.016 |
| 8 |
Ca2+ imaging in CB1−/− mouse PSN cultures |
115 |
74 |
41 |
From No 7: p < 0.0001 |
| 9 |
Ca2+ imaging in rat PSN cultures with a priori capsaicin application |
197 |
161 |
36 |
From No 1: p = 0.28 From No 2: p = 0.07 From No 10: p = 0.71 |
| 10 |
Ca2+ imaging in rat PSN cultures with a priori capsaicin application and rimonabant application |
237 |
190 |
47 |
From No 1: p = 0.4 From No 2: p = 0.11 From No 9: p = 0.71 |
| 11 |
Ca2+ imaging in rat PSN cultures with a priori capsaicin application and 1 μM NESS0237 application |
34 |
25 |
9 |
From No 1: p = 1 From No 2: p = 0.81 From No 9: p = 0.35 |
| 12 |
Whole-cell recordings from rat cultured PSN for single cell PCR |
56 |
31 |
25 |
From No 1: p = 0.08 From No 5: p = 0.8 From No 12: p = 0.79 |
| Single-cell polymerase chain reaction |
| Number of experiment |
Description of experiment |
CB1 receptor expression in ACR neurons |
CB1 receptor expression in COR neurons |
CB1 receptor expression in NR neurons |
Significant difference |
| 13 |
Single-cell PCR using RNA from rat cultured PSN |
11/11 |
8/10 |
4/8 |
n/a |
| Immunofluorescent staining |
| Number of experiment |
Description of experiment |
Total number of TRPV1-expressing cells |
Number of TRPV1 and CB1 receptor expressing cells |
Significant difference |
|
| 14 |
Immunofluorescent staining |
150 |
149 |
From number of capsaicin-responsive/CB1 mRNA expressing cells in No 12: p = 0.87 |
|
| Electron microcopy |
| Number of experiment |
Description of experiment |
Total number of membrane patches analysed |
Number of patches with high/medium receptor density |
Number of patches with low receptor density |
Significant difference from ACR/COR ratio |
| 15 |
Immuno- electron microcopy |
18 |
14 |
4 |
From No 1: p = 1 From No 2: p = 0.76 From No 9: p = 0.75 From No 12: p = 0.1 |
