Figure 3. Time-scaled Bayesian MCMC tree of HIV-1 B_CAR pol sequences from North America and Europe.

North American (n = 216) and European (n = 126) B_CAR sequences with known sampling date were combined with B_CAR sequences from the Caribbean (n = 258), B_PANDEMIC sequences (n = 50) the US and France and subtype D sequences (n = 10) from the DRC. Branches of trees reconstructed from subsets 1 (a), 2 (b), and 3 (c) are colored according to the most probable location state (see Table S3) of their descendent nodes as indicated in the legend (bottom left). Colored boxes indicate the positions of major (n ≥ 10) B_CAR clades detected outside the Caribbean. Branch lengths are depicted in units of time (years). The trees were automatically rooted under the assumption of a relaxed molecular clock. CA: Canada, CD: Democratic Republic of Congo, CEU: Central Europe, HISP: Hispaniola, JM: Jamaica, NEU: Northern Europe, SEEU: Southeastern Europe, SEU: Southern Europe, TT: Trinidad and Tobago, US: United States of America, WEU: Western Europe.