Product Name	Pkg. Size	Reconstitution	Working Concentration	Storage
Fibroblast Growth Factor-Basic (bFGF)	25 mg	To prepare 25 μg/ml stock solution; add 25 μg of FGF into 1 ml  of sterile medium (RPMI plus 1% FCS), swirl to dissolve, add 100 μl/aliquot	5 ng/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Stem Cell Factor (SCF)	10 mg	To prepare 10 μg/ml stock solution; add 10 μg of SCF into  1 ml  of sterile medium (RPMI plus 1% FCS), swirl to dissolve, add 250 μl/aliquot	5 ng/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Hepatocyte Growth Factor (HGF)	5 mg	To prepare 5 μg/ml stock solution; add 5 μg of HGF into  1 ml  of sterile medium (RPMI plus 1% FCS), swirl to dissolve, add 200 μl/ aliquot	2 ng/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Endothelin 3	50 mg	To prepare 50 μg/ml stock solution; add 50 μg of Endotelin into 1 ml of sterile medium (RPMI plus 1% FCS), swirl to dissolve, add 100 μl/aliquot	10 ng/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Laminin	1 mg	Thaw slowly at 2 - 8 oC, swirl and add 100 μl/aliquot	10 mg/ml	liquid -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Vascular Endothelial Growth Factor (VEGF)	10 mg	To prepare 5 μg/ml stock solution; add 10 μg of VEGF into  2 ml  of sterile water, swirl to dissolve, add 100 μl / aliquot	1 ng/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Leukemia Inhibitory Factor (LIF)	50 mg	To prepare 20 μg/ml stock solution; add 50 μg of LIF into  2.5 ml  of sterile water, swirl to dissolve, add 100 μl/aliquot	4 ng/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Adenine	25 g	To prepare 0.18 M stock solution; add 121.5 mg og adedine into 50 ml  of 0.05 M HCl (250 μl of 37.1% HCl into 50 ml of water)), swirl to dissolve, filter sterilize and add 1 ml/aliquot	1.8 x 10-3 M	powder 4 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Insulin	50 mg	To prepare 5 mg/ml stock solution; add 50 mg of insulin into 10 ml  of 0.005 M HCl (5 μl of 37.1% HCl into 10 ml of water)), swirl to dissolve, filter sterilize and add 0.5 ml/aliquot	5 mg/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
T3	100 mg	To prepare 2 x 10-8 M stock solution; add 3.4 mg of T3 into 25 ml  of 1 M NaOH, dilute 0.1 ml of this solution into 9.9 ml of PBS,  dilute again  0.1 ml of this solution into 9.9 ml of PBS, swirl to dissolve, filter sterilize and add 0.5 ml/aliquot	2 x 10-11 M	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Cholera Toxin	5 mg	To prepare 10-7 M stock solution; add 5 mg of Cholera toxin into 5 ml sterile ddH2O (store at -20 oC); dilute 50 μl of this solution into 5 ml ddH2O, swirl to homogenise, filter sterilize and add 0.5 ml/aliquot	10-10 M	powder 4 - 8 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Fibronectin	1 mg	To prepare 1 mg/ml stock solution; add 1 mg of fibronectin into 1 ml of sterile distiled water. Allow 30 min. for material to go into solution. DO NOT AGITATE OR SWIRL. Add 100 μl/aliquot	10 mg/ml	powder 4 - 8 oC, working aliquots -20 oC, avoid repeat freeze/thaw
apo-Transferrin	100 mg	To prepare 5 mg/ml stock solution (1,000x); add 100 mg of transferrin into 20 ml of PBS swirl to dissolve, filter sterilize and add 0.5 ml/aliquot	5 mg/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Heparin	50 KU	To prepare 50 mg/ml stock solution (1,000x); add 50 mg of heparin into 1 ml of water swirl to dissolve, filter sterilize and add 1 ml/aliquot	0.1 mg/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Heparan sulfate  proteoglycan	1 mg	To prepare 0.1 mg/ml stock solution; add 1 mg of heparan sulfate  into 10 ml of sterile water swirl to dissolve, and add 0.2 ml/aliquot	2 mg/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw
Collagen IV	5 mg	To prepare 2 mg/ml stock solutin: add 2 mg of collagen V into  2.5 ml of sterile 0.25% acetic acid. Allow 1 - 2 hr for material to go into solution.  Swirl for better dillution. Add 400 μl/aliquot	80 mg/ml	powder -20 oC, working aliquots -20 oC, avoid repeat freeze/thaw