Table 1.

The molecular intervention impact on cancer cell proliferation and metastatic capacity

Cancer cell line	Molecular intervention strategy	Changes of signalling pathway contributors after si/shRNA transfection	Impact on cancer function	Ref.
Hepato-cellular Huh7 cells	shRNA	Downregulation of Met, MMP2, MMP9 proteins expression	Reduction of cell migration and invasion abilities	[18]
Gallbladder cancer cells	siRNA	–	Reduction of cell proliferation, anchorage-independent growth, and cell migration	[22]
Pancreatic cancer CEPAC-1 cells	siRNA	Downregulation of Ras and ERK1/2 expression	Inhibition of cell proliferation, migration, and epithelial-mesenchymal transition Downregulation of MACC1 sensitised CFPAC-1 cells to gemcitabine	[23]
Naso-pharyngeal cancer CNE2 cells	siRNA	Inhibition of phosphorylated-Akt (Ser473) and β-catenin expression	Inhibition of cellular proliferation, migration, invasion, and colony formation Induction of apoptosis	[24]
Ovarian cancer OVCAR3 cells	siRNA	Reduction of Met protein expression	Downregulation of invasive, metastatic and angiogenic capacities of the cells	[25]
Ovarian cancer OVCAR3 cells	shRNA	Reduction of Met, MEK1/2, ERK1/2, cyclin D1 and MMP2 protein expression. Induction of cleaved caspase-3 level	Inhibition of cell proliferation, migration and invasion Induction of apoptosis	[26]
Cervical cancer SiHa cells	siRNA	Reduction of cyclin D1, Cdk2, MMP2, MMP9 proteins expression. Upregulation of p21 and E-cadherin protein expression	Suppression of cell proliferation, alteration of cell cycle distribution, reduction of cell invasion ability	[27]
Cervical cancer HeLa cells	siRNA	–	Reduction of cell proliferation or migration Induction of apoptosis	[28]
Osteo-sarcoma U2OS cells	siRNA	Inactivation of Akt signalling pathway	Inhibition of cell proliferation in vitro, colony formation, invasion and tumour growth in vivo Induction of apoptosis	[29]
Glioma U251 cells	shRNA	Cell cycle arrest at G1 phase The main regulatory targets: cyclins D1 and E Inhibition of enzymatic activities of MMP-2 and MMP-9	Inhibition of cell proliferation, invasion, and migration Enhancement of apoptosis	[30]