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. 2016 Sep 1;7(3):411–424. doi: 10.1016/j.stemcr.2016.08.002

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© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

In Vivo Contribution from B195AP⁺ Cells to Striated Muscle Regeneration

(A) Outline of experimental design.

(B) Histological analysis of the in vivo regenerative potential of grafted cells. (a) Tibialis anterior muscle section stained with H&E showing the localization of the other panels. (b and c) Regenerative muscle fibers, defined by centrally located nuclei (blue), showed EYFP expression, as detected with anti-GFP antibody (green). (d and e) Higher-magnification pictures of EYFP⁺ myofibers (green) and Laminin (red). Nuclei were counterstained with Hoechst 33258 (blue). Scale bars, 100 μm.