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Western blot and SDS-PAGE analysis of recombinant chick RALDH1, 2, and 3. (A–C) Recombinant RALDH1, 2, and 3 (500 ng) were immunoblotted with anti-chick RALDH2 (A), anti-chick RALDH3 (B), or anti-6His (C). (D) 500 ng of purified, recombinant RALDH1 (R1), RALDH2 (R2), and RALDH3 (R3) were run on a 1.0 mm 10% Bis/Tris gel. RALDH1, 2, and 3 (∼55–57 kDa) were greater than 90% pure, as determined by Coomassie Blue staining.
