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Western blot analysis and quantification of RALDH2 in control and 4-day recovering choroids. (A) Cytosol fractions from control (C) and day 4 recovering (R) choroids were immunoblotted with anti-chick RALDH2. RALDH2 immunopositive bands (∼55 kDa) were present in control and recovering choroids. (B) Average RALDH2 abundance (± SEM) in control and day 4 recovering choroids was measured as the IOD per 8-mm biopsy punch (n = 4). *P < 0.05 (paired t-test).
