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. 2016 Sep;57(11):4886–4897. doi: 10.1167/iovs.16-19395

Figure 6.

Figure 6

RALDH enzymatic activity in control and recovering chick ocular tissues using a HPLC/spectrophotometric assay for NAD-dependent atRA synthesis. (A) atRA synthesis is linear in untreated choroidal lysates from 0 to 60 minutes. All subsequent synthesis assays were conducted for 30 minutes. (B) Average RALDH enzymatic activity (± SEM) in chick ocular tissues in control and day 4 recovering eyes (n = 9). Activity was not detectible (N.D.) in the retina/RPE or sclera. (C) Average RALDH enzymatic activity (± SEM) in control and recovering choroids at various time points during the course of recovery (n = 6 measurements for each time point). Each activity measurement represents the average of two duplicate samples. *P < 0.05, **P < 0.01, ***P < 0.001 (paired t-test).