Figure 4. Western blot analysis of recombinant AtGPX1, 2, 3, 5, 6, and 8. Total soluble proteins were subjected to SDS-PAGE on 15% (w/v) gel, following the electrophoresis, proteins were transferred to PVDF membrane and AtGPXs proteins detected by polyclonal antibody raised by AtGPX8 (upper frame) or AtGPX6 (lower frame).