Fig 3. Neutrophils and macrophages.

(A) The number of neutrophils per mm² and (B) number of macrophages per mm² are shown in box graphs. Values are expressed as the mean ± SD, n = 5–6 for each group, ANOVA, Tukey-Kramer *p<0.05 (in black): the topical EB wound treatment group versus the subcutaneous E2 pellet group, *p<0.05 (in red): the topical EB wound treatment group versus the topical E2 skin application group. (C) Neutrophils (arrows) stained with an anti-neutrophil antibody and macrophages (arrows) stained with an anti-Mac-3 antibody were observed in wound tissue on day 7. Bar, 20 μm.