Fig 4. Neutrophil recruitment to the corneas of infected GF SW mice is increased at the peak of infection.

Ocular surface washes were collected from P. aeruginosa-infected GF (n = 5) and SPF SW (n = 5) mice. 5 μg total protein was in-gel digested, trypsinized, labeled, and profiled using LC-MS/MS. Significantly upregulated proteins in the infected GF mice were visualized using String-based web software and revealed enrichment for proteins associated with phagocytosis (FDR 0.000525), phagosome (FDR 0.003), leukocyte trafficking (FDR 0.02) and high degree of protein-protein interaction (PPI enrichment p value 7.55e-15).