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Bioluminescence emission spectra (400–750 nm) produced by the LUCs at 25°C (A) pH 5.5 and (B) pH 7.8, respectively. Enzyme (50 μg) was assayed with 1.5 ml mix buffer containing 0.47 mM luciferin, 1.0 mM ATP, 10 mM MgSO4, 10 mM DTT and 25 mM tricine, adjusted to pH 7.8 and 5.5, respectively.
