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. 2016 Sep 22;11(9):e0162983. doi: 10.1371/journal.pone.0162983

Fig 5. Mild acid hydrolysis of EPS.

Fig 5

(A) Separation of EPS fragments by PGC HPLC with MS/MS detection. The extracted ion chromatogram for mass 1008.39 Da shows four peaks. Their reducing end sugar was clearly revealed by ESI-MS/MS. Their assignment as either Gal or Glc and the interpretation in terms of fragment structures was done a posteriori based on MALDI-TOF data and on knowledge of the EPS structure. (B) Example of a MALDI-TOF/TOF fragment spectrum showing b-ions from the non-reducing and y- and y´ (= 1,5x) -ions from the reducing end.