Fig 4. Augmentation of cell death by autophagy inhibitors.

(A) SAS cells were treated with 10 μM safingol and 1 mM 3-MA or 5 nM bafilomycin A1 for 24 h and cell viability was measured by the MTT assay. Data were means±SD (n = 3), *P<0.05. (B) SAS treated with safingol and 3-MA or bafilomycin A1 for 24 h were analyzed using flow cytometry and the proportion of viable cells, early apoptotic cells, late apoptotic cells, and necrotic cells were determined.