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. 2016 Sep 22;11(9):e0163359. doi: 10.1371/journal.pone.0163359

Fig 3. PolyQ expansion in Ataxin-1 and Htt N548 proteins increases S-nitrosylated and S-acylated high molecular weight (HMW) species.

Fig 3

SNO-RAC and acyl-RAC were used to recover S-nitrosylated and S-acylated proteins followed by Western blotting. (A) Top: S-nitrosylated and S-acylated monomer and high molecular weight (HMW) species of Ataxin-1 expressed in HEK293T cells. Bottom: HMW/monomer quantity ratios for Ataxin-1Q85 (n = 5). All experiments show that HMW material is enriched in S-nitrosylated and S-acylated proteins. (B) S-nitrosylated and S-acylated monomer and HMW species of Htt N548 expressed in HEK293T cells.