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. 2016 Sep 22;11(9):e0163359. doi: 10.1371/journal.pone.0163359

Fig 6. Co-expression of nitric oxide synthase (NOS) promotes Htt N548 inclusion formation.

Fig 6

EGFP-tagged Htt N548 fragments were expressed in HEK293T cells for two days. Empty vector co-expression serves as the negative control by comparison to nNOS, eNOS, or iNOS. (A) Representative fluorescence microscopy images of co-expression of NOS and N548 showing the fluorescent inclusions that are present. All images were acquired under the same parameters. (B) Htt N548Q15 and N548Q128 inclusions are adjacent to the nucleus. A single focal plane (2 μm thickness) is shown. The DAPI signal is shown in red and EGFP-tagged N548 signal in green. (C) The percentage of cells with N548Q15 inclusions in the presence of NOS expression. Top: average of three experiments; bottom: individual experiment (n = 3). (D) The percentage of cells with N548Q128 inclusions in the presence of NOS expression (n = 3). Top: average of three experiments; bottom: individual experiments. For quantification, we divided the number of cells with inclusions by the total number of cells expressing EGFP signal. The error bar represents the SEM.